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KMID : 0368420070500060636
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Journal of Plant Biology
2007 Volume.50 No. 6 p.636 ~ p.643
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Nitric Oxide Elicitation Induces the Accumulation of Secondary Metabolites and Antioxidant Defense in Adventitious Roots ofEchinacea purpurea
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Wu Chun-Hua
Tewari Rajesh Kumar
Hahn Eun-Joo
Paek Kee-Yoeup
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Abstract
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Nitric oxide and reactive oxygen species are important signal molecules that play key roles in plant defense responses. We investigated the involvement of nitric oxide elicitation in the synthesis of secondary metabolites within the adventitious roots ofEchinacea purpurea. When roots were treated with 100 μM sodium nitroprusside (SNP), an exogenous nitric oxide producer, the accumulation of phenolics, flavonoids, and caffeic acid derivatives was enhanced. This level of SNP also induced an antioxidant defense, as indicated by increases in Superoxide dismutase, ascorbate peroxidase, and ascorbic acid, along with decreases in hydrogen peroxide, lipid peroxidation, and dehydroascorbate/ascorbic acid. However, a higher concentration (250 μM SNP) acted as a pro-oxidant, thereby raising the levels of hydrogen peroxide, lipid peroxidation, and dehydroascorbate/ascorbic acid while diminishing ascorbic acid, ascorbate peroxidase, and the accumulation of secondary metabolites compared with our observations at 100 μM SNP. Therefore, we conclude that elicitingE. purpurea adventitious roots with a concentration of 100 μM SNP is beneficial to their accumulation of secondary metabolites.
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KEYWORD
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antioxidant defense, bioreactor, Echinacea purpurea, elicitation, nitric oxide, secondary metabolites
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